"We have never used it to enhance performance"
In a statement issued to Road.cc, the team maintained that its use of the method was strictly diagnostic: “The INEOS Grenadiers did not use this method in the 2024 racing season or seasons before that – for the avoidance of doubt, that includes the 2024 Tour de France."
"The team has since used this as a diagnostic tool – this has only ever been used as a measurement method to check how individual riders respond to altitude (this can be simulated or natural altitude) as well as heat stress and training," INEOS continued. "We have never used it to enhance performance and have always adhered to
UCI rules and regulations on this issue.”
INEOS Grenadiers team poses during a team presentation at the 2025 Tour de France
Why is CO2 rebreathing controversial?
The testing, according to The Times, involved seven riders — some reportedly teenagers — inhaling a mixture of oxygen and carbon monoxide for five to six minutes, followed by a short rest and then a second test. While experts cited in the report noted that repeated testing in one session can be standard scientific practice to establish an average value, the procedure's timing raised eyebrows.
The second round of tests took place on February 1 — the very same day the World Anti-Doping Agency (WADA) announced new measures restricting carbon monoxide rebreather use. From February 10, the UCI introduced a rule limiting CO inhalation to a single test per two-week period, with usage only permitted under strict medical supervision for diagnostic purposes.
The report also states that the tests were overseen by a professor from the Inland University in Lillehammer, Norway, who has since joined Q36.5, another UCI-registered team. Sources told The Times that not all participating riders had been involved in altitude training at the time, and one reportedly experienced nausea and breathing difficulties during the back-to-back sessions.
INEOS’ acknowledgement comes amid a wider reckoning in professional cycling over the ethics, legality and potential performance-enhancing effects of carbon monoxide rebreathing, a technique originally developed in the 1980s to measure total haemoglobin mass (Hb-mass). While long considered a valid diagnostic tool, concerns have emerged over whether repeated usage could stimulate erythropoiesis — the production of red blood cells — thus mimicking the physiological adaptations of altitude training.
WADA has now included non-diagnostic use of carbon monoxide inhalation on its 2026 Prohibited Methods list, citing evidence that the method can induce hypoxia and increase erythropoietin production under certain conditions. The UCI’s ban already covers repeated use, and mandates medical oversight for all diagnostic applications.