However, if anyone can turn the tables it's the two most recent winners of the rainbow jersey. They won't be riding in their INEOS team colours, Ganna will represent Italy while Foss is part of the Norwegian team in Montreal.
Sunday's time trial will encompass 39 kilometres of largely flat terrain before a 400 metre climb to the finish at an average gradient of six percent - adding a final moment of suspense for fans and riders as differences could be made in the final push to the line.
Ahead of the big day, we've looked back on INEOS's three-year domination in the discipline before Remco Evenepoel took the baton and ran with it.
2022: Tobias Foss
When Tobias Foss won the 2022 World Championships, it certainly turned a few heads. Although a strong time triallist, the Norwegian wasn't expected to be in the medals when the dust settled in Wollongong.
He finished two seconds clear of Stefan Kung and nine seconds ahead of Evenepoel when the final results came through. However, a tricky course lent itself to suiting his credentials.
The 34 kilometre course was largely flat, but featured a technical opening half before a faster final run-in. He was 11 seconds behind Kung and four ahead of Evenepoel at the second timing point, but surged in the final minutes to deny Kung and keep Evenepoel at bay in an emphatic finale.
The Montreal course features several technical sections as well as its fair share of long and exposed straits this time around, drawing some comparisons to that 2022 time trial.
Filippo Ganna, a powerhouse against the clock
2021 & 2020: Filippo Ganna
If Remco Evenepoel is the best time trial rider of his generation, Filippo Ganna is certainly the second best, and he's got two world titles to prove it. Back in 2020 and 2021, it seemed like the Italian was unbeatable.
He has won 33 time trials across his storied career, and the 30-year-old won his first world title back in 2020 at the Imola World Championships. On that occasion, he bested Wout van Aert and Stefan Kung comfortably, putting 29 and 36 seconds into the pair respectively.
It was much of the same the following year at the Leuven World Championships. Van Aert was once again in second, but this time six seconds adrift. Evenepoel took third place but 44 seconds adrift.
The INEOS powerhouse looked in fine form at the Tour of Britian at the beginning of the month and the time trial has been on his radar since he finished the Tour de France in July.
He and Evenepoel have traded wins in head-to-head time trials against eachother, can Ganna roll back the clock and add a third rainbow jersey to his palmares?